The bilayer technique is a method to record electrical currents at a single channel level. The bilayer forms a gigaohm resistance between two saline-buffer filled chambers. After incorporation of pore forming proteins (ion channels, solute channels, carriers and pumps) protein mediated currents or membrane potentials can be recorded at high resolution
In membrane biology, electrophysiology is one of the central investigation tools. In the past the standard technological approach has primarily consisted of the various alterations of patch clamping of membranes and cells. However, certain limitations with respect to flexibility, throughput, user friendliness, and information content have become obvious over the years.