The HEKA Fitmaster features analysis and fitting routines for electrophysiological data. Analysis can be performed on the levels of Sweeps/Traces and Series. Besides standard fit functions such as Polynomials, Exponentials, Gaussians, and Boltzmanns, tailored functions to fit e.g. whole-cell current traces according to Hodgkin & Huxley gating formalism, current-voltage relationships and dose-response curves allow publication-proof analysis of your data.
The experienced patch clamp investigator will be fascinated by the versatility of the program and logical structure of the functions. All of the features of PATCHMASTER’s Online Analysis are also present in the HEKA Fitmaster. In principle, the HEKA Fitmaster extends these capabilities by adding more specific analysis features and the generation of an analysis tree.
The way the HEKA Fitmaster works is tightly linked to the tree structure of data generated by PATCHMASTER up to the level of a Series. Hence, the HEKA Fitmaster has equivalent hierarchical levels of analysis corresponding to the tree levels featured in the Replay data tree. The analysis is organized in a structured way, relating to Sweeps and Series. The lowest level is the analysis of a raw data trace, a Trace. The TraceFit dialog provides tools for selecting a section of the trace for analysis and specifications of the type of analysis to be performed.
The values determined by this sweep analysis can be further processed in SeriesFit. But in contrast to PULSEFIT, HEKA Fitmaster allows pooling of data on the level of the Series by introducing the new waves buffer concept. Several results of the TraceFit can be compiled in the waves buffer and then displayed in the SeriesFit graph. Several fit functions can be used to describe this ensemble of data points.
FITMASTER now features Action Potential analysis …
… and Single Channel detection.
Runs on Windows 7, 8 and on Mac OS X > 10.6 (requires a free USB port for the dongle)
- Current-voltage curve analysis
- Inhibition and activation analysis
- Dose-response curve fitting
- Analysis of evoked and spontaneous currents and potentials
- Action potential analysis
- Single channel analysis
- Amplitude histograms
- Power spectra analysis
- Fit to raw data traces and analysis results
- Fitting to user defined functions