The D330 MultiStim System, available here at Digitimer, has been designed for use in laboratories where a number of in vitro preparations or cell culture wells need stimulation from a low impedance source. These studies are known as punctate or field/bath stimulation.
The Multistim System uses a common timing waveform for each channel but a different stabilised stimulus to each preparation. The Multistim includes modules for constant voltage stimulation up to 100v 1a (d333h) and constant current stimulation up to 500ma from a 100v source (d343).
The D330 Multistim System is available in a 19” rack or bench mountable case, to suit the individual requirements of our clients and customers, which can house up to 10 channels of stimulation. The system is modular so that a minimal system may be expanded upon at a later date or split into smaller units for two sites.
Modules are available to generate pulses variable in frequency and duration; these can be supplied in bursts using a gating waveform variable in repetition, duration or count. In addition, extra delayed pulses can be generated for studies involving refractory period. Most controls on the D330 Multistim System are either single turn or 10-turn with locking dials. Sockets are fitted to allow external control and synchronisation.
Contact us by calling us on 01707 328347 or by emailing us at firstname.lastname@example.org if you wish to learn more information about the D330 MultiStim System. The experienced, knowledgeable Digitimer team will be able to answer any questions you have to do with our full collection of products.
Do You Need Help with MultiStim System Module Selection?
The resources below are provided to assist you in module selection and system configuration, but if you still need advice, please contact us.D330 MultiStim Module Selection Flowchart
D330 MultiStim Operating Modes
D330 MultiStim Brochure
- High power system for field stimulation of tissue and organ baths systems.
- Timing parameters of each stimulation channel can be completely & independently controlled through the use of external logic control.
- External devices can be used as a trigger or gating signal to all channels within the stimulator.
- Internal pulse and train generation can be provided by selection of appropriate modules.
- BiStim module allows normal, reverse or alternating output polarity.
- High power output of up to 100V from constant voltage or 0-500mA from constant current sources.
- Meter module allows monitoring of individual channel outputs.
- Additional delayed pulse enables studies of effective refractory period to be carried out using the MultiStim.
|Case & Power Supply Unit|
|D337||19" Case & Power Supply Unit.|
|Pulse Generation & Timing Modules|
|D331AT Sync & Gate Module||Sync & Gate Module - Allows internal control of timed bursts of pulses.|
|D332T Pulse Module||Pulse Module - Provides duration and frequency control of stimulation pulses.|
|D334B Sync Module||Sync Module - Useful in configurations where there is some degree of external timing control.|
|D340 Count & Delay Module||Count & Delay Module - Provides additional delayed pulse which can be used to determine effective refractory period.|
|D341A Sync & Train Module||Sync & Train Module - Allows internal control of a repetitive burst of "x" pulses.|
|D333H Dual Stimulator Module (Constant Voltage)||Dual Stimulator Module (Constant Voltage, 2 channels per module) - 0 to 100V with a maximum of 1A.|
|D343 Dual Stimulator Module (Constant Current)||Dual Stimulator Module (Constant Current, 2 channels per module) - 0 to 500 mA from 100 V.|
|D342 Dual Bistim Module||Dual Bi-stim Module - Alternate output polarity of D333H or D343 using relays.|
|D335 Meter Module||Meter Module - This module allows the output (current or voltage) of any channel to be monitored.|
|D344 Remote Module||Remote Module - Provision for complete external control of timing through TTL inputs from a computer interface.|
|D330-x Blanking Panels||Blanking Panels x-wide (where x can be 1,2,5,7 module widths). For safety reasons, blanking panels must be fitted to all unused bays in D337 Case.|
1. I am having difficulty deciding what modules I will need to carry out my multi-channel stimulation experiments, do you have any advice?
Of course, feel free to contact us to discuss your requirements, but perhaps you should take a look at our comprehensive D330 operating mode notes and module selection flowchart (see above) before doing so.
2. I want to stimulate a set of organ baths, how many can I stimulate using the D330 system?
A single D337 rack supports 2 -10 channels of stimulation. In addition, multiple D337 racks can be linked by a “sync” cable allowing this maximum to be increased further. Please remember that for safety reasons any empty bays must have blanking panels fitted to protect the user from possible electric shock.
3. I want to make up some electrode leads to connect to a MultiStim. What size are the plugs and where can I get them?
The output sockets on the MultiStim fit 2mm plugs. We can supply these in packs of 10 with either black or red insulation (part number NL822P/10-Blk or NL822P/10-Red).
4. I want to use my computer & data acquisition interface to provide the timing controls for the stimulation output pulses – How can I do this?
This is the simplest possible MultiStim setup. If your software and hardware is capable of producing the required stimulation timing characteristics (i.e pulse frequency and duration), then all you require is a D337 Case, D334B Sync Module, the required number of dual stimulator modules/blanking panels and an appropriate cable (usually Lemo plug to BNC plug) to link the interface to the MultiStim. We also recommend a D335 meter in order to easily monitor the output current.
5. I have re-arranged my dual stimulator modules in the case and now the stimulators display a lit error LED on the front panel?
The D337 Case is specifically designed to hold timing and pulse generator modules in the first (left-hand) four bays. Dual width stimulator modules (D333H or D343) will ONLY operate correctly if they are placed in the remaining 10 bays.
6. When I increase the output amplitude on my stimulator modules during stimulation, the overload light comes on – what’s happening?
The D333H and D343 modules include an overload LED which serves to warn the user if the current requested or required cannot be supplied by the stimulator. The D333H modules have an upper limit of 1A, so if the impedance of the preparation and the voltage settings are high enough, the module will reach the maximum output current and indicate an overload. The same effect can be seen with the D343 except this is a constant current device and will supply up to 500mA until it reaches a compliance limit of 100V. If you run into the above problems, consider whether you need to have the output settings so high and also check that your preparation and electrodes provide a low impedance connection.