AutoMate Scientific design and manufacture a range of application specific electrophysiology recording chambers which are fully compatible with their perfusion systems. Their current range includes the new QuickStage Microscope Stage Adapters and Heated Perfusion Chambers, the OPC-1 Oocyte Perfusion Chamber, specifically designed for two electrode voltage clamp studies of Xenopus oocytes, and the PCP-1 Petri-dish Perfusion Chamber, which is QuickStage compatible and ideal for use with cultured cells in 35mm dishes. Many AutoMate chambers can be used with their 4 into 1 or 8 into 1 inert micromanifolds, which are available as optional items.
Oocyte Perfusion Chamber (OPC-1)
Enhance your study of oocyte recombinant receptor expression with a small working volume (<20µl) Xenopus oocyte perfusion chamber for fast solution exchange in automated and unattended experiments. It includes an integrated outflow tube and agar bridge for easy setup.
Expression of recombinant receptors in oocytes has been a favorite choice for researchers to assess the pharmacology of recombinant receptors. Since the oocytes are 1-1.5 mm in diameter, however, fast solution exchange around the oocyte was difficult. After several years of electro-physiological research, we have designed a Xenopus oocyte perfusion chamber for use in automated and unattended experiments. Combined with an automated perfusion system, this chamber allows researchers to obtain dose-response data quickly and easily.
- Small working volume (<20ul)
- Fast Solution Exchange
- Optional 4 into 1 or 8 into 1 PTFE Micro-manifold
- Available with 80, 90, 100, 108, 110 or 120 mm Stage Adaptors
Petri-dish Perfusion Chamber (PCP-1)
Why transfer to coverslips? Perfuse cells right in your 35mm Petri dish or six-well plates. The AutoMate Scientific Petri Dish Chamber works with any perfusion system and optional PTFE inert micromanifold. It is ideal for inverted microscopy using optically clear Petri dishes. An adjustable metal suction tube is included for continuous inflow and outflow.
Cells cultured in Petri dishes are a popular research tool used in numerous applications, including patch clamping and intracellular ion probe imaging. True perfusion (continuous inflow and outflow) of solutions can be difficult to configure. This forces scientists to plate cells on cover slips for placement into specially designed perfusion chambers. However, such transfer is a time consuming process which introduces the potential for contamination plus additional expense. The PCP-1 chamber was designed by scientists after years of patch clamp research to overcome this problem. Perfuse cells right in your Petri dish with any perfusion system and an optional PTFE inert manifold (sold separately). Ideal for inverted microscopy using optically clear Petri dishes. Adjustable metal suction tube included. Dimensions: 35mm, outside diameter x 20mm tall. AutoMate’s Petri Dish Perfusion Chambers work well with MatTek’s 35mm Glass Bottom Culture Dishes, allowing researchers to produce high-resolution microscopic images of cultures growing in a standard-size 35mm disposable plastic Petri dish.
- Available for Corning, Falcon and Nunc Dishes (please specify when ordering)
- Compatible with optional 4 into 1 or 8 into 1 PTFE micro-manifold
- Adjustable stainless steel aspirator tube included
Quickstage Microscope Stage Chambers
QuickStage™ Microscope Stage Adapters and Heated Perfusion Chambers from AutoMate Scientific. QuickStage is designed to fit directly into your Olympus, Nikon, Zeiss (K & M), Leica, Siskiyou, Scientifica, Narishige, Thor Labs, or Sutter microscope stage or platform. With a simple twist of two locking nuts, the entire stainless magnetic Tool Tray lifts off with all of your magnetic tools still attached. The underlying chamber and coverslip are then free for easy cleaning and replacement. Optical #1 thickness cover slips provide a perfect imaging surface for upright or inverted microscopy.
- Integrated spill channel
- Magnetic stainless steel tool platform
- For Nikon, Olympus, Zeiss and Leica upright & inverted microscopes
The heated version of the QuickStage uses AutoMate Scientific’s popular ThermoClamp temperature controller to maintain your set temperature from ambient to 50°C. Disassembly and cleaning of the heated chamber is just as simple as the unheated version. The ferro-stainless Tool Tray can be removed with all of your tubes and electrodes intact. A silicone gasket ensures leak-free performance without messy grease. Stage heating is maintained while coverslips are replaced so you can begin recording again sooner.
QuickStage part numbers look complicated, but they are actually pretty easy. Refer to the chart below:-
Heated Perfusion Chambers
Unheated Perfusion Chambers
Unheated perfusion chambers: horizontal, round, and vertical (QS-U-H, -V & -R) These use 22 mm square #1 coverslips on the bottom, and accept 13 mm round coverslips on top. Small magnets and clips hold your pipes. These work with any of our stage adapters.
Microscope Stage Adaptors
Tool Trays are ferro-magnetic stainless steel plates designed to hold your perfusion chamber down to the stage adapter while also providing a large surface for StageHandsTM magnetic tool holders. Round stage adapters (i.e. Olympus and Nikon) include a square Tool Tray (4.25 in / 10.8 cm wide x 4.56 / 11.6 cm deep). Rectangular stage adapters (i.e. Zeiss and Universal) include a rectangular Tool Tray (5 in. / 12.7 cm x 3.5 in / 8.9 cm deep).
If you don’t have room on your stage for a Tool Tray, or think it is too large, you can order your QuickStage with Clamps instead. They hold the perfusion chamber down to the stage adapter using the same two thumb nuts on each side. For heated cell chambers (not unheated chambers or 35 mm dish holders), Clamps also require four small screws to hold the chamber, gasket and coverslip to the heating adapter.
Microscope Selection Chart
Heated Environmental Lid for 35mm dishes
Heated glass lid with perfusion and gas control attaches magnetically to the top of our 35mm dish holders. Control the temperature of a large 30 mm coverslip lid to minimize condensation while maintaining gas and temperature control over your 35mm dish. Separate perfusion inflow, aspiration outflow, gas connection, temperature sensor and grounding provided.
Dual-channel ThermoClamp®-2 Temperature Controller
Two independent channels for simultaneous heating of Environmental Lid or Perfusion Pencil® inline heater and heated QuickStage.
35mm Petri Dish Holders and Heaters
Unheated 35mm Petri dish holder (QS-U-35) with Nunc adapter ring. Rotating spring clips hold your dish securely for long-term imaging. This adapter also holds full- size slides. Shown with Tool Tray on Zeiss “M” stage adapter.
Adapters available for Nunc, WillCo, Falcon, In Vitro (D35-10-1-N), Corning, Mattek, and In Vitro (D29-10-1-N) 35mm dishes. A heated 35mm Petri dish holder (QS-H-35) with spring clips and Corning adapter ring if also available with integrated temperature sensor (see below).
StageHands™ Magnetic Tool Holders
Magnetic base, arms, manifold and tube holders mount everything securely on your microscope stage for electrophysiology and perfusion. The magnetic base of these different StageHands are de
signed to stick tightly to a steel ring around your perfusion chamber like the QuickStage™, or any steel surface nearby.
They include a 1⁄4-20 screw to insert into a non-magnetic platform or air table. Footprint: 0.75″ (19 mm), adjustable in all axes, extends to over 4.5″ (12 cm) long. Can be used to hold electrodes, tubes, manifolds, temperature sensors, glass capilliaries, agar bridges, etc. Thumbscrews allow easy adjustment without needing any tools. All stainless steel and anodized aluminum parts so they won’t oxidize or corrode.
Start with the StageHands Kit #1 (U.SH-1 shown, tool tray not included) which comes with two magnetic bases with a pair of 1.5″ (4 cm) arms, one locking ball joint, both a single and double tube/ electrode holder, plus a separate manifold holder. Add more arms, holders or bases as desired.