The D380 Iontophoretic Dye Marker provides an isolated, bipolar and constant current output intended for cell labelling or cell marking of individual neurons with charged dyes, such as Lucifer Yellow, via iontophoresis. Holding currents of up to ±6nA and injection currents up to ±12nA are possible and precisely set by single-turn panel mounted dials. A compliance voltage in excess of ±6V allows the full injection current into electrodes with impedances up to 500Mohms. An LCD screen continuously displays the polarity and magnitude of the requested Injection and Holding currents as well as the electrode resistance and the voltage applied. The unit is powered from a single, standard, 9 Volt battery.
A novel feature of the unit is that the injection current polarity can be easily reversed by a push button switch to facilitate electrode de-blocking.
- Holding current of ±6nA
- Injection current of ±12nA
- Polarity reversal for de-blocking purposes
- For pipette resistances of up to 500Mohms.
- LCD Display screen
1. Is it possible to control the delivery of dye using an external device?
The D380 included a TTL-gate BNC input socket, which allows a TTL high input to activate the injection current. This means that external devices can be used to control the timing of current delivery.
2. When should I replace the battery?
The 9V battery will last a long time (approx. 100 hours continuous use), however, if you start to see “Compliance Error” on the display, this is an indication that the voltage available is no longer adequate to pass the requested current. At this point, either the electrode impedance is higher than normal or the battery is failing.
3. How do I connect my electrode to the D380?
The D380 is not supplied with any connection cables for the pipette/electrode, so we recommend you fabricate this link using a low leakage coaxial cable. The core should be connected to the pipette containing the dye and the shield should be in electrical contact with the bathing medium so that the circuit is completed and current can flow.
D30 Iontophoretic Dye Marker
Publications which cite use of the Digitimer D380 can be found on Google Scholar.